Animal Parasitology Lab. Biol 4426L

Lab. #10

Nematodes

PHYLUM NEMATODA (Introduction Text pages 355-384)

Subclass Adenophorea

Order Trichuridae

Family Trichuridae

Trichuris trichiura – (Text pages 385-387)

Slide: Female whole mount

- What is morphologically distinctive about the anterior end of this worm?

- What distinctive cells can be seen in the esophagus?

Slide: Male whole mount

- What differences can you see in the male and female organs of this worm?

- Make sure your drawing includes the details of the male organs of this worm.

Slide: eggs

- What distinctive features are visible on each end of this egg?

Family Trichinellidae (Text pages 389-395)

Trichinella spiralis -

Slide: Male and female whole mounts

Draw: Whole adult female

- Locate the anterior and posterior of this worm.

- What organs can you see inside the female?

- Where is this life cycle stage found in the host?

Draw: whole adult male

- What is the size of this worm relative to the female?

- Locate the copulatory bursa

- What internal organs can you see?

- Where is this life cycle stage found in the host?

Slide: encysted in muscle

- Locate the larval worms inside the tissue

- Identify the nurse cell surrounding the worms

- What is this life cycle stage ?

Subclass Secernentea

Order Rhabditida

Family Strongyloididae

Strongyloides stercoralis –(Text pages 399-403)

Slide: parasitic female

- What is the general shape of the body?

- What is the shape of the esophagus?

- What is the shape of the tail?

Slide: free living female

- What is the general shape of the body? How does this compare with the parasitic female?

- What is the shape of the esophagus?

- What structures are visible inside the body that tell you this is the female?

Slide: free living male

- What is the general shape of the body?

- What is the shape of the tail?

- What structure near the end of the tail distinguishes this as a male?

Slide: Rhabditiform larvae

- What is the shape of the esophagus?

- What is the general shape of the body

- What is the size relative to the free-living males and females?

- What is the shape of the tail?

Slide: Filariform larvae

- What is the shape of the esophagus?

- What is the general shape of the body?

- What is the size relative to the rhabditiform larvae?

- What is the shape of the tail?

What is unusual about the life cycle of this worm?

What are the differences in morphology of the free living forms and the parasitic forms?

Order Oxyurida

Family Oxyuridae

Enterobius vermicularis - –(Text pages 433-436)

Slide: Female whole mount

- What is the shape of the esophagus?

- What is the shape of the tail? How does it differ from the male tail?

Slide: Male whole mount

- What is the shape of the esophagus?

- What is the shape of the tail? How does it differ from the female tail?

Slide: Eggs – what single character distinguishes this egg?

What is the common name of this worm?

How can you become infected with it? Who is most likely to be infected?