Animal Parasitology Lab. Biol 4426L

Lab. #11.

Order Strogylida

Family Ancylostomatidae (Text pages 405-412)

Ancylostoma duodenale - (Text pages 408-409)

Slide: male –

Draw: whole worm and enlargement of tail

- What is the morphology of the tail?

- How does it compare with that of Necator (see below)

- What is the morphology of the mouth? How does it compare with that of Necator?

Slide: female

Draw: whole worm and enlargement of mouth

- find the ovary, eggs if present

- What is the morphology of the tail?

- What is the morphology of the mouth?

Slide: Rhabditiform larvae

- what is the shape of the esophagus?

- Where is this life cycle stage found/

- Is this the infective stage?

Slide: Filariform larvae

- what is the shape of the esophagus?

- Where is this life cycle stage found/

- Is this the infective stage?

What is the common name of this parasite?

How does a person become infected by this worm?

Where can it be found in the world?

Family Uncinariidae

Necator americanus – (Text pages 407-408)

Slide: Adults (females or males)

- What is the morphology of the mouth?

Slide: hookworm eggs

- How many cells of the developing embryo can be seen?

- What does the shell look like?

What is the common name of this parasite?

How does a person become infected by this worm?

Where can it be found in the world?

Order Ascaridida

Family Ascaridae

Ascaris lumbricoides – (Text pages 419-423)

Slide: males and females in cross section (Draw a separate picture of each)

- Locate the uterus and/or ovaries of the female

- Locate the testes in the males

- What is the diameter of the sections relative to the sex?

- Also locate the dorsal and ventral nerve chords, intestine, pseudocoel, cuticle

Whole worm

- What is the relative size of the different sexes?

- How many lips are present?

- How can you distinguish males from females (besides size?)

Slide: eggs

- What character distinguishes this egg?

What are the two main hosts for this worm?

Toxocaris canis – (Text pages 423-425)

whole worms - Observe this worm under the dissecting microscope and make a drawing of the anterior end.

What age group of dogs and humans are most susceptible to infection by this worm?

Order Spiruida

Family Onchocercidae - Filarial worms

Onchocerca volvulus – (Text pages 452-456)

Slide: Tissue section of "tumor" containing adult worms

- Locate the sections of worms

- Locate the “tumor” tissue

What is the vector of this worm?

Where are the adults found?

Dirofilaria immitus – (Text pages 452-456)

Model: heartworm in dog heart

Slide: smear with microfilariae

- What are the cells on the slide?

- What is the sice of the microfilaria in relation to these cells?

What is the vector of this worm?

Where are the adults found?

Where are the microfilariae found?

Acanthocephala

Phylum Acanthocephala (Text pages 469-482)

Class Eoacanthocephala

Order Neoechinorhynchidea

Family Neoechinorhynchidae

Within the species below make sure you draw a picture of at least one male and one female.

Neoechinorhynchus sp. – (Text pages 476-477)

Slide: whole mount

- What is the shape of the proboscis? How many hooks can be see on it?

- What internal organs can be identified?

- What sex is of the specimen you have?

- Locate sexual organs and identify them

Class Palaeacanthocephala

Order Echinorhynchidea

Family Rhabdorhynchidae

Leptorhynchoides thecatus –

Slide: whole mount

- What is the shape of the proboscis? How many hooks can be see on it?

- What internal organs can be identified?

- What sex is of the specimen you have?

- Locate sexual organs and identify them

Eocollis sp. –

Slide: whole mount

- What is the shape of the proboscis?

- What distinctive feature is seen on the proboscis of this parasite?

Class Archiacanthocephala

Order Oligacanthorhynchidea

Family Oligacanthorhynchidae

Macracanthorhynchus hirudinaceus –

Slide: tissue section

- What structures of the acanthocephalan can you identify in the slide provided?

- What structures of the host intestine can you identify in the slide?

- Can you see the leucocytic infiltration caused by this parasite?

- What is the usual host for this parasite?

Slide: Acanthocephalan eggs

- How many layers of membranes can you see?

- Be able to distinguish this egg from all the others.

Slide: Unknown species of Acanthocephalan

- Examine the slide provided and try to identify the parasite based on the information you have learned during lab.

MORPHOLOGICAL FEATURES OF ACANTHOCEPHALANS

During your examination of these parasites try to identify and label the following morphological features: