ANIMAL PARASITOLOGY LAB. BIOL 4426L

LAB. #2.

Protozoa

Phylum Sarcomastigophora - flagellates and amoebas

Subphylum Mastigophora - Flagellates

- Introduction, morphology (Text pages 53-55)

Trypanosoma brucei gambiense - (Text pages 55-59)

Slide: trypomastigotes in blood

- Find the parasites in the blood smear. How big are they? (Measure 5-10 parasites) How big are they relative to the red blood cells?

- Locate and identify: undulating membrane, kinetoplast, nucleus, flagellum

Study questions: What disease does this parasite cause?

What is the geographic distribution of this disease?

How does this disease differ from the disease caused by Trypanosoma brucei rhodesiense?

What is the vector of these diseases?

What is the method of infection?

Trypanosoma cruzi (Text pages 62-66)

Slide: trypomastigote in blood

- Find the parasites in the blood smear. How big are they? (Measure 5-10 parasites) How big are they relative to the red blood cells?

- Locate and identify: undulating membrane, kinetoplast, nucleus, flagellum

- How big is the kinetoplast in relation to the body of the cell? - How big is the kinetoplast in relation to the one seen in Trypanosoma brucei gambiense?

- How can you distinguish this parasite from Trypanosoma brucei gambiense?

Slide: amastigote form in muscle

- Locate and identify the host tissue

- Locate pseudocysts with amastigote forms inside. How do these differ from the trypomastigote form?

Study questions: What disease does this parasite cause?

What is the geographic distribution of this disease?

What is the vector of this disease?

What is the method of infection?

What pathological effects does it have on man?

Leishmania donovani - (Text pages 54 and 68-73)

Slide: promastigote form from culture

- What is the size of the parasite?

- What is the position of the kinetoplast relative to the nucleus?

- Can you find an undulating membrane?

- Compare this form with the trypomastigote forms studied above.

Chilomastix mesnili - (Text pages 81-82)

Slide: trophozoites and cysts

- Find the trophozoites in the fecal smear. How big are they? (Measure 5-10 parasites)

- Locate and identify: nucleus, flagella, cytosomal cleft.

- Locate the cyst. What is its shape. How big are they relative to the trophozoite?

- Identify the cyst wall, nucleus, cytosomal cleft, flagella.

Study questions: Where in man is this protozoan found?

Does it cause disease?

Why is it important to be able to identify it?

Giardia lamblia - (Text pages 82-85)

Slide: trophozoites and cysts

- How many nuclei does the trophozoite have? How many flagella?

In the trophozoite, locate and identify the adhesive disk, nuclei. median body(ies), axonemes, flagella.

- In the cyst, locate the nuclei, median body(ies). How many are there of each? What is the overall shape of the cyst?

Study questions: What animals are the hosts for this parasite?

The non-human hosts for this parasite are called what?

How do you get infected with this parasite?

Trichomonas vaginalis - (Text pages 86-89)

Slide: trophozoites

- Locate and identify the nucleus, undulating membrane, anterior flagella, axostyle.

- What color is the parasite in this stained preparation?

Study questions: Where is this protozoan found in men, in women?

Does it cause disease?

How do you get infected with this parasite?

Where can we find the cyst forms of this parasite?

Pentatrichomonas hominis -

Slide: trophozoites

- Locate and identify the nucleus, undulating membrane, anterior flagella, axostyle.

How does this protozoan differ from Trichomonas vaginalis?

Study questions: Where is this protozoan found? Does it cause disease?