ANIMAL PARASITOLOGY LAB. BIOL 4426L

LAB. #3.

Protozoa

Phylum Sarcomastigophora - flagellates and amoebas

Subphylum Sarcodina - Amoebas - Introduction (Text page 99)

Entamoeba histolytica - (Text page 99-105)

Slide: trophozoites

- Find the amoebas in the slide. How big are they?

- Locate and identify the nucleus, nuclear endosome, periferal chromatin material. Where is the endosome located inside the nucleas (in the middle or offset to one side)?

- Can you see vacuoles in the cytoplasm? If so can you recognize any of the contents?

Slide: cysts

- Find the cysts in the slide. How big are they?

- How many nuclei are present?

- Locate chromatoidal bars. How many are present in the cyst? What is their shape?

Slide: intestinal tissue section

- Identify the host tissue.

- Locate the parasites.

- What kind of damage can you see to the host tissue?

Study questions: What form (or stage) of this parasite is infective to man?

How does this parasite cause disease?

How can a person become infected with this parasite?

How can transmission of this disease be prevented?

Entamoeba coli - (Text page 105-106)

Slide: trophozoites

- Find the amoebas in the slide. How big are they?

- Locate and identify the nucleus, nuclear endosome, periferal chromatin material.

- Where is the endosome located inside the nucleas (in the middle or offset to one side)?

- What is the thickness of the periferal chromatin material as compared to that of Entamoeba histolytica?

- Can you see vacuoles in the cytoplasm?

Slide: cysts

- Find the cysts in the slide. How big are they?

- How many nuclei are present?

- Locate chromatoidal bars. How many are present in the cyst? What is their shape?

- Can you see vacuoles in the cytoplasm?

Study questions: How is this amoeba distinguished from Entamoeba histolytica?

Is this amoeba parasitic? Does it cause disease?

Where in the host is it found?

Why is it important to distinguish it?

Endolimax nana - (Text page 107)

Slide: trophozoites and cysts

- Find the trophozoites in the slide. How big are they?

- Locate and identify the nucleus, nuclear karyosome.

- Where is the karyosome located inside the nucleus (in the middle or offset to one side)? How big is it compared to the endosome in Entamoeba histolytica?

- Is periferal chromatin material present?

- Can you see a vacuoles in the cytoplasm?

- How many nuclei are present in the cysts?

- Are chromatoidal bars present in the cyst?

- Can you see vacuoles in the cytoplasm?

Study questions: Where in the host is it found?

How is this amoeba distinguished from Entamoeba histolytica and Entamoeba coli?

Is this amoeba parasitic?

Iodamoeba buetschlii - (Text pages 107-108)

Slide: trophozoites and cysts

- Find the trophozoites in the slide. How big are they?

- Locate and identify the nucleus, nuclear endosome.

- Where is the endosome located inside the nucleas (in the middle or offset to one side)? How big is it compared to the endosome in Entamoeba histolytica?

- Is periferal chromatin material present?

- Can you see vacuoles in the cytoplasm?

- Find the cysts in the slide. How big are they?

- How many nuclei are present?

- Are chromatoidal present in the cyst?

- Can you see vacuoles in the cytoplasm? What is the size of any vacuole relative to the size of the cyst?

Study questions: Where in the host is it found?

How is this amoeba distinguished from Entamoeba histolytica and Entamoeba coli?

Is this amoeba parasitic?

Phylum Ciliophora (Text page 174)

Balantidium coli (Text pages174)

Slide: cysts – demonstration

What is the size of the macronucleus?

Can you find the micronucleus?

Slide: tissue section of intestine

Find the parasite in the tissue.

Can you distinguish cilia? Find the cytostome (vestibule)

Study questions: Where in the host is it found?

Is this ciliate parasitic?

What other animals are host for this parasite?

Ichthyophthirius multifiliis (Text pages 175-176)

Slide: tissue section of skin

Find the parasite in the skin

What is the shape of the macronucleus?

Study questions:

What are the principal hosts of this organism?

What is the major feature that distinguishes this organism?

What is the life cycle of this organism?

Trichodina sp. (Text pages 176)

Slide: Gill smear

What is the shape of the parasite?

What unusual structure is present in the cell that gives the cell rigidity?

Study questions:

Is this a parasite?

How does this parasite harm its host?