ANIMAL PARASITOLOGY LAB. BIOL 4426L

LAB. #4.

sufficient material for the time alCoccidians

Phylum Apicomplexa

Class Sporozoasida

Subclass Coccidiasina

Order Eucoccidiorida

Family Eimeriidae

Eimeria tenella - (Text pages 120-121)

Slide: intestinal tissue section

- Orient yourself to the cellular structure of this tissue.

- Identify the intestinal villi, basement layers and muscularis

- Locate infected epithelial cells, distinguish them from uninfected cells.

- Draw cells containing developing schizonts, microgmetes, and

macrogametes. Can you identify merozoites

- Locate and identify oocysts in the lumen

Study questions: What animal is affected by this parasite?

Where in the host are the various stages of this parasite found?

What stages in the life cycle can you identify in the slide provided?

What stages in the life cycle are NOT found in the slide?

Family Sarcocystidae

Toxoplasma gondii - (Text pages 123-127)

Slide: intestinal tissue section

- Identify the intestinal villi, basement layers and muscularis

- Locate infected epithelial cells

- Draw cells containing developing schizonts, microgmetes, and

macrogametes. Can you identify merozoites

- Locate and identify oocysts in the lumen

Study questions: What is the definitive host for this parasite?

How does this life cycle differ from that of Eimeria?

How do humans become infected? (two methods)

Which host, definitive or intermediate, is represented in this slide?

How does this parasite affect man?

Family Plasmodiidae

Plasmodium vivax thin smear - (Text pages 140-145 and Plate 1)

Slide: blood smear

Locate and draw a red blood cell with a signet-ring trophozoite

Locate and draw a red blood cell with a mature schizont

In what host is the sexual life cycle of the parasite completed?

What stages can you find in the blood of humans?

Plasmodium falciparum - (Text pages 145 and Plate 3)

Slide: blood smear

Locate and draw a red blood cell with a signet-ring trophozoite

Locate and draw a red blood cell with multiple infections of the

signet-ring trophozoites

How does this form of malaria differ from the vivax form?

What stages are found in peripheral blood of humans?

What stage in the blood of humans is distinctive for this species?

Slide: blood smear with gametocytes

Locate and draw a red blood cell with a macrogametocyte

Locate and draw a red blood cell with a microgametocyte

UNKNOWN CLASSIFICATION

Pneumocystis carnii - (Text pages 131-133)

Slide: human lung section

How many people are infected with this parasite?

Why do AID's patients succumb to this disease?

DEFINE: Sporogony - Merogony -Gametogony -