ANIMAL PARASITOLOGY LAB. BIOL 4426L

                                                           LAB. #8.

 

                                                  Flatworms

 

 

Phylum Platyhelminthes

          Class Cestoidea(Text pages 297-323)

                    Order Pseudophyllidea

                              Family Diphyllobothriidae (Text pages 325-329)

 

Diphyllobothrium latum – (Text pages 325-329)

 

Slide: scolex

          -Locate and identify the attachment organs on the scolex.  What is this type of attachment called?

 

Slide : proglottid

          -Locate the ovary, testes, uterus and genital opening

 

Slide: eggs

          -Can you see the operculum and the small knob at the terminal end?

 

 

Taenia solium – (Text pages 333-335)

 

 Slide: Scolex with hooks

 

-        Locate and identify the attachment organs on this worm

-        There are other species of  cestodes that you will see that have a similar attachment organs. 

-        Be able to distinguish the scolices

 

Slide: mature proglottid – demonstration

 

-        Locate the ovary, uterus, genital opening, cirrus, testes, vitelline gland

 

Slide: gravid proglottid - demonstration       

 

          -    How many branches of the uterus can you count.  How many are there supposed to be?

 

Slide: Cysticercus whole mount

 

-        Locate the invaginated scolex.  Make sure you see the rostellar spines

-        What is the common name of this type of larval cestode?

 

 

 

Taeniarhynchus saginatus – (Text pages 331-333)

 

Slide: mature proglottid

 

-        Locate the ovary, uterus, genital opening, cirrus, testes, vitelline gland

 

Slide: gravid proglottid -

 

          -    How many branches of the uterus can you count.  How many are there supposed to be?

 

Slide: Eggs

 

-        What is distinctive about these eggs?  These are typical for all the species of Taenia.

 

 

Echinococcus granulosis – – (Text pages 337-340)

 

Slide: whole worm

 

-        How many proglottids are present on this worm?

-        What kind of attachment organs are present on the scolex

-        How big is the whole worm?

 

Slide: hydatid sand

 

-        Where are these parasites found?

-        What host?

-        Locate the rostellar spines

 

Slide: hydatids in tissue sections

 

-        Locate the parasite tissue.  How many layers can you see?

-        Identify the developing protoscolices

-        What host is this stage found in?

 

Slide: Eggs

 

-        What is distinctive about these eggs?  Can you distinguish these from the Taenia eggs?