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Juan López Garriga, Ph.D.

Physical Chemistry: Protein Structure*

Email: sonw@caribe.net

Office: Q-134

Ext. 2107

Research Interests:

Structure and function relationships in hemeproteins, like the unique hemoglobin I from Lucina pectinata. For example, using “site directed mutagenesis” we tailor the structure of this hemoglobin. Its chemical structure is then studied by Fourier Transform infrared, resonance Raman vibrational analysis, and NMR spectroscopies. This is followed by the kinetic study of the reaction between hemoglobin and ligands (for example, O2, CO, NO, and H2S) using time – resolved (pump and probe) infrared and resonance Raman techniques. Ultrafast geminate chemical dynamics are studied also in our laboratory using time - resolved (pump and probe) picosecond and femtosecond spectroscopy.

Research Project
The project involves the use of the unusual hemoglobin from the tropical clam L. pectinata as a model system to study the relationship between chemical structure, reactivity and function of heme proteins with hydrogen peroxide.

Representative Publications:

C. Ramos, R. Pietri, W. Lorenzo, E. Roman, L.B. Granell, C.L. Cadilla, J. López-Garriga, Recombinant hemoglobin II from Lucina pectinata: a large-scale method for hemeprotein expression in E. col, The Protein Journal, 2010, 29(2), 143 – 151.

Carlos A. Nieves-Marrero, Carlos R. Ruiz-Martínez, Rafael A. Estremera-Andújar, Luis A. González, Juan López-Garriga and Jose A. Gavira. Two-Step Counterdiffusion protocol for the crystallization of Hemoglobin II from Lucina pectinata in the pH range 4 to 9. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2010, F66, 264-268.

C. Bonaventura, R.W. Henkens, W. De Jesus-Bonilla, J. López-Garriga, , Y. Jia, A.  Alayash, C. Parker, A.  Crumbliss., Extreme Differences between Hemoglobins I and II of the Clam Lucina pectinalis in Their Reactions with Nitrite. BBA - Proteins and Proteomics, 2010, 1804(10), 1988-1995.

E. Román-Morales, R. Pietri, B. Ramos, S.N. Vinogradov, J. Lopez-Garriga,  Structural determinants for the formation of sulfhemeprotein complexes. Biochemical and Biophysical Research Communications. (2010)  In Press.

Carlos R. Ruiz-Martínez, Carlos A. Nieves, Rafael A. Estremera, José A. Gavira, Luis A. González-Ramírez, Juan J. López-Garriga and Juan M. García-Ruiz. Crystallization and Diffraction Patterns of the Oxy and Cyano Forms from the Lucina pectinata Hemoglobins Complex. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2009, F65, 25-28.

R.Pietri, A. Lewis, R.G. León, G. Casabona, L. Kiger, S.R. Yeh, S. Fernandez-Alberti, M.C. Marden, C.L. Cadilla, and  J. López Garriga, “Factors Controlling the Reactivity of Hydrogen Sulfide with Hemeproteins”, Biochemistry, 2009, 48,  4881-4894.

R., Pietri, León, R.G., Kiger, L., Marden, M. C., Bacelo, D.,  Binning Jr. R. Cadilla, C. L., López-Garriga, J.  Ligand-binding control in HemoglobinI from L. pectinata: Structural implications of B10, E7 and E11 mutantsBiochemistry, 2009, 48, 4881 - 4894.

O'Neill, E., Collins, D., López-Garriga, J., Macchiavelli, R., Cruz, J., Center for Resources in General Education (CIVIS): Towards Student Success in General and STEM Education. ASEE/IEEE Frontier in Education 2009, TIA-A7.

C. Ruiz, C., Nieves, C., Estremera, R., Lopez-Garriga, J., Jimenez, J. Ciencia sobre ruedas en Granada : Una experiencia de actualización científica y tecnológica en el aula. Enseñanza de las Ciencias, Investigación en Didáctica de las Ciencias, 2009, 1319-1322.

E. Ramírez, A. Cruz, D. Rodríguez, L. Uchima, R. Pietri, A. Santana, J. López Garriga, G. E. López, "Effects of active site mutations in haemoglobin I Lucina pectinata: a molecular dynamic study”, Molecular Simulations, 2008, 34(7), 715-725.

L. Rivera, J. Lopez-Garriga, and C.L. Cadilla, "Characterization of the full length mRNA coding for L. pectinata HbIII revealed an alternative polyadenylation site". Gene Volume 2008, 410 (1), 122-128.

José A. Gavira, Ana Camara Artigas, Walleska De Jesús Bonilla, Juan López Garriga, Ariel Lewis, Ruth Pietri, Syun-Ru Ye, Carmen L. Cadilla, and Juan Manuel García Ruiz, “Structure and ligand selection of hemoglobin II from Lucina Pectinata”, Journal of Biological Chemistry, 2008, 283(14), 9414-9423.     

Angelines Castro Forero, David Jimenez, Juan López Garriga, Madeline Torres, “Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks”, Journal of Applied Polymer Science, 2008, 107(2), 881-890.

S. Fernández-Alberti, D. E. Bacelo, R. C. Binning Jr. J. Echave, M. Chergui, and J. López-Garriga, Sulfide-Binding Hemoglobins:  Effects of Mutations on Active-Site Flexibility.  Biophysical Hournal 2006, 91, 1698-1709.

Ruth Pietri, Ruth G. León, Laurent Kiger, Michael C. Marden, Laura B. Granell, Carmen L. Cadilla, Juan López-Garriga, Hemoglobin I from Lucina pectinata:  A model for distal heme-ligand control, Biochimica et Biophysica Acta – Proteins & Proteonomics, 2006, 1764 (4), 758-765.

José A.  Gavira, Walleska de Jesús, Ana Cámara-Artigas, Juan López-Garriga and Juan M. García Ruíz.  Capillary crystallization and molecular-replacement solution of haemoglobin II form the clam Lucina pectinata.  Acta Cryst Section F, 2006, F62, 196-199.

Ruth Pietri, Laura Granell, Anthony Cruz, Walleska De Jesús, Ariel Lewis, Ruth Leon, Carmen L. Cadilla and Juan López Garriga, Tyrosine B10 and heme–ligand interactions of Lucina pectinata hemoglobin II: control of heme reactivity, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 2005,  1747(2),  195-203.

Evidence for nonhydrogen bonded compound II in cyclic reaction of hemoglobin I from Lucina pectinata with hydrogen peroxide. De Jesus-Bonilla, Walleska; Ramirez-Melendez, Eunice; Cerda, Jose; Lopez-Garriga, Juan. Chemistry Department, University of Puerto Rico, Mayaguez, P. R. Biopolymers (2002), 67(3), 178-185.

Expression and purification of recombinant hemoglobin I from Lucina pectinata. Rosado-Ruiz, Tanya; Antommattei-Perez, Frances M.; Cadilla, Carmen L.; Lopez-Garriga, Juan. Chemistry Department, University of Puerto Rico, Mayaguez, P. R. Journal of Protein Chemistry (2001), 20(4), 311-315.

Formation of Compound I and Compound II Ferryl Species in the Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide. De Jesus-Bonilla, Walleska; Cortes-Figueroa, Jose E.; Souto-Bachiller, Fernando A.; Rodriguez, Lolita; Lopez-Garriga, Juan. Chemistry Department, University of Puerto Rico, Mayaguez, P. R. Archives of Biochemistry and Biophysics (2001), 390(2), 304-308

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