Meléndez’s research work entails the design of metal-based drugs and organometallic biosensors. New metallocene drugs which target specific tumors and receptors are developed as tools for treating cancers. The mechanistic aspects of these species are studied by spectroscopic methods and molecular modeling. We examine the binding of drugs to biomolecules such as proteins and oligonulceotides using a wide variety of physical and analytical techniques such as CD, UV-Vis, fluorescence and 2D NMR spectroscopies and electrochemical methods. To explain the experimental results, we use molecular modeling methods.
Another project in collaboration with the Material and Engineering Department is the development of electrochemical biosensors. The principal objective of this work is to develop an electrochemical biosensor based on conducting polymers electrode functionalized with ferrocene to detect substrate such as glucose and breast cancer.
“Molybdeonoce-oligonucleotide binding study at physiological pH using NMR spectroscopy and cyclic voltammetry”J.L. Vera, F.R. Román and E. Meléndez Biorg. Med. Chem. 2006, 14, 8683.
“Determination of the titanium content in human transferrin by Inductively Coupled Plasma-Atomic Emission Spectroscopy” A. Cardona and E. Meléndez Anal. Bioanal. Chem. 2006 386(6), 1689.
“Synthesis, Solution and Solid State Structure of Titanium-Maltol Complex” J. L. Lamboy, A. Pasquale, A.L. Rheingold and E. Meléndez Inorg. Chim. Acta 2007, 360, 2115.
“Synthesis, Ti(IV) intake by apotransferrin and cytotoxic properties of functionalized titanocene dichlorides” L-M. Gao, R. Hernández, J. Matta and E. Meléndez J. Biol. Inorg. Chem. 2007, 12(7), 959-967.
“Host-Guest Interactions between Cp2NbCl2 and α-, b-, γ-Cyclodextrins: Preparation and Characterization in Solution and Solid State” A. Morales, J. Struppe, and E. Melendez. J. Inclusion Phenomena and Macrocyclic Chemistry 2008, 60, 263-270.
“Structure-Activity Studies of Ti(IV) Complexes: Aqueous Stability and Cytotoxic properties in colon cancer HT-29 cells”R. Hernández, J. Lamboy, L.M. Gao, J. Matta, F. R. Román1 and E. Meléndez. J. Biol. Inorg. Chem. 2008, 13, 685-692.
“Spectroscopic and Thermal Characterization of Host-Guest Interactions α-, β-, and γ-cyclodextrins and vanadocene dichloride. A. Morales, R.T. Weber and E. Meléndez. App. Organometal. Chem. 2008, 22, 440-450.
“Synthesis, Structure, Electrochemistry and Cytotoxic Properties of Functionalized Ferrocenes” L.M. Gao, R. Hernández, J. Matta and E. Meléndez. Metal Based Drugs 2009, Article ID 420784.
“Water-soluble molybdenocene complexes with both proliferative and antiproliferative effects on cancer cell lines and their binding interactions with human serum albumin” I. Feliciano, J. Matta and E. Meléndez. J. Biol. Inorg. Chem 2009, 14(7):1109-17
“Synthesis, Structure and Biological Activity of Amide-Functionalized Titanocenyls: Improving their Cytotoxic Properties” L.M. Gao, J. Matta, A. Rheingold and E. Meléndez. J. Organometal. Chem. 2009, 694, 4134.
“Titanium(IV) Complexes : Cytotoxicity and Cellular Uptake of Titanium(IV) Complexes on Caco-2 Cell Line” Ramón Hernández1,3, Janet Méndez2, José Lamboy1, Madeline Torres2, Féliz R. Román1 and Enrique Meléndez1. Toxicology in Vitro 2009, doi:10.1016/j.tiv.2009.09.010.